Purified Anti-Human CD23 Antibody[EBVCS2] (E-AB-F1382A)
![Purified Anti-Human CD23 Antibody[EBVCS2] - 1](http://file.elabscience.com/assets/images/loading.png)
For research use only.
Conjugation: Unconjugated
APC Elab Fluor®700 Elab Fluor®Violet 450 FITC PE PE/Cyanine 7 PerCP/Cyanine 5.5 Unconjugated Show More
| Alternate Names | alpha polypeptide, Blast 2, Blast2, C type lectin domain family 4 member J, CD 23, CD 23A, CD23 antigen, CLEC 4J, C-type lectin domain family 4, Fc epsilon receptor II, Fc fragment of IgE, Fc fragment of IgE low affinity II receptor for, Fc fragment of IgE receptor II, Fc of IgE, Fc receptor, Fc receptor IgE low affinity II alpha polypeptide, FCE 2, FCER 2, FCER2A, FceRII, IgE, IgE binding factor, IgE receptor lymphocyte, IGEBF, IgE-binding factor, Immunoglobulin E binding factor, Immunoglobulin E receptor, Immunoglobulin epsilon chain, isoform CRA_a, LEUKOCYTE ANTIGEN CD23, Low Affinity IgE Receptor, low affinity II, Low affinity immunoglobulin epsilon Fc receptor membrane bound form, Low affinity immunoglobulin epsilon Fc receptor soluble form, Ly42, Ly-42, Lymphocyte antigen CD23, member J, MGC93219, receptor for (CD23), FCE, FCER, BLAST, BLAST-2, CD23, C-type lectin domain family 4 member J, Fc epsilon RII, Fc-epsilon-RII, Immunoglobulin E-binding factor, Low affinity immunoglobulin epsilon Fc receptor, Lymphocyte IgE receptor, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Fc&, epsilon-RII-I, ILT9, ILT10, BLAST-2 |
| Clone | |
| Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells) |
| Background | CD23 (also named B cell differentiation antigen) is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily. Human CD23 is a 47 kDa type II transmembrane glycoprotein that is expressed by a wide variety of cell types. The full-length receptor is 321 amino acids (aa) in length and contains a 274 aa extracellular region, a 26 aa transmembrane segment, and a 21 aa cytoplasmic domain. The extracellular region contains a C-type lectin domain and a connecting stalk with coiled-coil topography. The lectin domain binds both protein and carbohydrate in an apparently Ca++ independent manner. The coiled-coil region contributes to oligomerization. The lectin domain in human CD23 (aa 162‑284) is 64%, 62% and 68% aa identical to the lectin domains in mouse, rat and bovine CD23, respectively. In the cytoplasmic region, two FC isoforms exist which arise from alternate start sites. The “a” (or long) isoform begins with the sequence MEEGQYS and is constitutively expressed by B cells. It is believed to participate in IgE-mediated endocytosis. The “b” (or short) isoform begins with MNPPSQ and is induced on a wide variety of cell types by IL-4. Fcb reportedly contributes to IgE-mediated phagocytosis (13). Fcb expressing cells include eosinophils, monocytes, visceral smooth muscle and intestinal epithelium. At least four soluble forms of CD23 are known to exist. They range in molecular weight from 25 kDa to 37 kDa, with the 25 kDa form predominating in sera. Soluble CD23 (sFc) is generated by metalloprotease (ADAM8; ADAM15; ADAM28) and cysteine-protease activity. Cleavage usually occurs between aa 150‑160. It is unclear if sequential metalloprotease-cysteine protease activity is necessary for the generation of all soluble forms. Both soluble and membrane-bound CD23 show bioactivity. Ligands for CD23 include CD21, IgE, CD11b, and CD11c. CD23 binding to CD11b and Cd11c on monocytes results in oxidative product generation and proinflammatory cytokine release. On B cells, sCD23 induces IgE secretion by binding CD21. Conversely, secreted IgE will, in turn, bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production. |
| Immunogen | Recombinant Human CD23 protein |
| Abbre | CD23 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG1, κ |
| Isotype Control | Purified Mouse IgG1, κ Isotype Control[MOPC-21] |
| Applications |
FCM
|
| Research Areas | Immunology;Stem Cells;CDs;Hematopoietic Stem Cell Markers |
| Cellular Localization |
Secreted, Cell membrane
|
| Tissue Specificity |
Detected in urine (at protein level).
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | Unconjugated |
| Storage Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
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